Oxidants, antioxidants, and the beneficial roles of exercise-induced production of reactive species.

This review offers an overview of the influence of reactive species produced during exercise and their effect on exercise adaptation. Reactive species and free radicals are unstable molecules that oxidize other molecules in order to become stable. Although they play important roles in our body, they can also lead to oxidative stress impairing diverse cellular functions. During exercise, reactive species can be produced mainly, but not exclusively, by the following mechanisms: electron leak at the mitochondrial electron transport chain, ischemia/reperfusion and activation of endothelial xanthine oxidase, inflammatory response, and autooxidation of catecholamines. Chronic exercise also leads to the upregulation of the body's antioxidant defence mechanism, which helps minimize the oxidative stress that may occur after an acute bout of exercise. Recent studies show a beneficial role of the reactive species, produced during a bout of exercise, that lead to important training adaptations: angiogenesis, mitochondria biogenesis, and muscle hypertrophy. The adaptations occur depending on the mechanic, and consequently biochemical, stimulus within the muscle. This is a new area of study that promises important findings in the sphere of molecular and cellular mechanisms involved in the relationship between oxidative stress and exercise.

Evaluation of hydration status following soccer matches of different categories / Avaliação de parâmetros de hidratação após jogos de futebol de diferentes categorias

The purpose of this study was to evaluate the hydration status and thermoregulatory responses, during official soccer matches, of soccer players in different categories. The participants of the under-15 (U-15, n=36) and of the under-17 category (U-17, n=14) were placed into different groups according to the amount of time spent in the field: Main group; Partial group; Intermediate group; Control group. The thermoregulatory responses and hydration status were measured. The Main group and the Partial group presented significantly higher water intake, weight loss and sweat rate compared with the Intermediate and Control group (p< 0.05). The under-17 players of the Main group had a larger weight difference pre and post match compared with the under-15 players of the same group (p< 0.05). It was concluded that an official soccer match altered significantly the hydration status of the players, and it was related with the time spent in the match.

Objetivo do presente estudo foi avaliar o padrão de hidratação e respostas termorregulatórias de jogadores de futebol de diferentes categorias ao longo de um jogo oficial. Participaram desse estudo jogadores de futebol das categorias sub-15 (N 36) e sub-17 anos (N 14). Os jogadores foram divididos em grupos de acordo com o tempo de atuação em nos jogos: grupo principal, o grupo parcial, grupo intermediário grupo controle. As respostas termorregulatórias decorrentes dos jogos e o estatus de hidratação foram determinados e comparados entre os grupos apresentados. Os grupos principal e parcial apresentaram maiores valores de consumo de água, perda de peso e taxa de sudorese em comparação aos grupos intermediário e controle (p< 0,05). A diferença de peso pré e pós-jogo entre as categorias foi maior nos atletas da categoria sub17 em comparação com os atletas sub 15 no mesmo grupo (p< 0,05). Conclui-se que um jogo de futebol foi capaz de alterar de forma significativa os parâmetros de hidratação dos jogadores de futebol tendo relação direta com o tempo de atuação em campo.

Impact of heat and pollution on oxidative stress and CC16 secretion after 8 km run.

To investigate the acute effect of a hot, humid and ozone-polluted (O(3)) environment on lung inflammation and oxidative tress of runners performing 8 km time trial run. Using a single-blinded randomized design, 10 male athletes (mean[Formula: see text]= 64.4 mlO(2) kg(-1) min(-1), SD = 4.4) took part in a time trial run in four different environmental conditions: 20°C + 50% relative humidity (rh) (Control); 20°C + 50% rh + 0.10 ppm O(3) (Control + O(3)); 31°C + 70% rh (Heat); 31°C + 70% rh + 0.10 ppm O(3) (Heat + O(3)). Blood samples and nasal lavage were collected post-exercise and analyzed for inflammatory, epithelial damage and oxidative stress markers. Data were analyzed using repeated measures ANOVA with Tukey's post hoc test. A significant increase in CC16 concentration (P < 0.05) and GSH/protein concentration (P < 0.05) in the upper respiratory airways was observed following the 8 km run in the Heat + O(3) trial compared with the control trial. There were no differences in the neutrophil counts between trials. No differences were observed for the other antioxidants analyzed. A hot, humid and ozone-polluted environment (0.1 ppm) elicits an early epithelial damage and antioxidant protection process in the upper respiratory airways of athletes immediately after performing 8 km time trial run.

Investigating performance and lung function in a hot, humid and ozone-polluted environment.

Large urbanized areas, where sports events take place, have a polluted environment and can also reach high temperatures and humidity levels. The aim of this study was to investigate the impact of a hot, humid and ozone-polluted (O(3)) environment on (1) performance of an 8 km time trial run, (2) pulmonary function, and (3) subjective respiratory symptoms in endurance-trained runners. Using crossover randomized design, 10 male participants (mean V(O)2(max)= 64.4 mlO(2) kg(-1) min(-1), SD = 4.4) took part in a time trial run under four different conditions: 20 degrees C + 50% relative humidity (rh) (Control), 20 degrees C + 50% rh + 0.10 ppm O(3) (Control + O(3)), 31 degrees C + 70% rh (Heat), 31 degrees C + 70% rh + 0.10 ppm O(3) (Heat + O(3)). Heart rate, ratings of perceived exertion and minute ventilation were collected during the run. Lung function was measured pre and post-exercise. The runners completed a respiratory symptoms questionnaire after each trial. The completion time of both the Heat (32 min 35 s) and Heat + O(3) (33 min 09 s) trials were significantly higher (P < 0.0001) when compared to the Control + O(3) (30 min 27 s) and Control (30 min 15 s) trials. There were no significant changes between pre/post lung function measures or between trials. The effective dose of ozone simulated in the present study did not affect the performance and therefore, ozone-pollution, at an environmentally relevant concentration, did not compound the impairment in performance beyond that induced by a hot, humid environment.